Protein G Sepharose Fast Flow is recombinant protein G coupled to Sepharose 4 Fast Flow resin.
- High binding capacity: approximately 20mg human IgG per mL resin
- Broad-spectrum: binds to a broad range of IgG species and subclasses
- Complements Protein A resins: unlike protein A, protein G binds all human and mouse IgG subclasses, including human IgG3 and mouse IgG1 as well as all rat IgG subclasses
- No specific albumin binding: recombinant protein G has been genetically engineered to remove the albumin binding region
- Minimal leaching: optimized coupling method minimizes leaching of recombinant protein G and improves binding capacity for IgG
- Wide range of formats: including loose resin packs from 5mL to 5L, for manual or automated antibody purification and immunoprecipitation procedures
IgG purification
Protein G Sepharose 4 Fast Flow is a good first choice for general-purpose capture of antibodies at laboratory scale. Compared to protein A-based resins, it binds a broader range of immunoglobulins from eukaryotic species and more classes of IgG.
Key applications include:
- isolation and purification of IgG from serum, ascites, and tissue culture media
- monoclonal antibody purification
- isolation of antibody-ligand immune complexes
- immunoprecipitation protocols
The recombinant protein G ligand binds strongly to the Fc-region from a variety of mammalian species. In addition, many antibodies also interact via the Fab region with a low affinity site on protein G. The ligand is immobilized via a multipoint coupling to the Sepharose 4 Fast Flow agarose matrix to minimize ligand leakage.
Protein G Sepharose 4 Fast Flow resin is part of Cytiva's BioProcess media (resins) range, which ensures security of supply and comprehensive technical and regulatory support.
This product has met the following criteria: