Application

Dilute formamide according to specific application requirements. Formamide concentrations of 45-50% are typically used in Southern and Northern analyses and a 50% or greater concentration for in situ hybridization techniques. In DNA to DNA hybridization, each 1% increase in formamide concentration lowers the Tm by 0.7%.

Research CategoryAll

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

This product is intended for use in hybridization applications for in situ, Southern, Northern, dot and slot blot analyses, and colony, plaque, and YAC screening. Formamide is used primarily as a component in probe hybridization solutions to promote probe hybridization specificity. It denatures nucleic acids by disrupting hydrogen bonds.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

This preperation of formamide is deionized, triple distilled and packaged under nitrogen and is free of detectable DNAse, RNAse, phosphatase, and protease activity.

Quality

Each lot of material is pre-filtered through a mixed-ion exchange resin to eliminate products of decomposition and residual metal cations. This filtering process guarantees that each lot has a final purity of greater than 99.5% and an adsorbance value of less than 0.05 adsorbance units at 280nm. Each lot then undergoes rigid quality control testing to insure that assay specifications are met prior to release.

Storage and Stability

Store at -20°C. Keep cap tightly closed after opening or aliquot and store at -15°C to -25°C.