Brilliant Blue G, 250, for microscopy

Code: 27815-25G-F D2-231

Analysis Note

λmax. ~610 nm; E(1%,1 cm) ~500-700 (H2O)

Application

Brilliant Blue G was used to prepare the protein re...


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£77.00 25G
£92.40 inc. VAT

Analysis Note

λmax. ~610 nm; E(1%,1 cm) ~500-700 (H2O)

Application

Brilliant Blue G was used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery.

Biochem/physiol Actions

P2X7 purinergic receptor antagonist.

General description

Brilliant Blue G (BBG) is a dye. It has been proposed as a substitute for internal limiting membrane (ILM) peeling.

Other Notes

Protein stain for gel electrophoresis. Does not penetrate the gel and is three times more sensitive than Naphthol Blue Black B; Simple and sensitive protein assay; Rapid, sensitive and versatile assay for proteins; free amino acids and several chemicals that interfere with the Lowry procedure cause no interference.

Quality Level200
Quality Level200
SMILES stringO.Cc1ccc(cc1)S(O)(=O)=O
SMILES string[Na+].CCOc1ccc(Nc2ccc(cc2)C(c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5C=CC(C=C5C)=[N+](CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
impurities8-12% water (Karl Fischer)
impurities≥85% toluene-4-solfonic acid
assay≥99% (calc. to H2O free subst.)
InChI1S/C7H8O3S.H2O/c1-6-2-4-7(5-3-6)11(8,9)10;/h2-5H,1H3,(H,8,9,10);1H2
InChI1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1
ign. residue≤0.5%
InChI keyKJIFKLIQANRMOU-UHFFFAOYSA-N
InChI keyRWVGQQGBQSJDQV-UHFFFAOYSA-M
gradefor microscopy
Cas Number6104-58-1
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