Analysis Note

Count the number of colonies on both sides of the paddle. CASO Agar with TTC: Count the number of colonies for obtaining the total aerobic bacterial count (TVC). Rose Bengal Chloramphenicol Agar: Count the number of colonies to obtain the fungal contamination (colonies of yeasts are red-pink)

Sterility: passes testpH-value/side one (25 °C): 6.6 - 7.3pH-value/side two (25 °C): 6.2 - 7.0Growth/side one - Escherichia coli ATCC 8739 (WDCM 00012): good - Staphylococcus aureus ATCC 6538 (WDCM 00032): fair/good - Candida albicans ATCC 10231 (WDCM 00054): none/poor - Saccharomyces cerevisiae ATCC 9763 (WDCM 00058): none/poor - Pseudomonas aeruginosa ATCC 9027 (WDCM 00026): good - Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053): goodGrowth/side two - Escherichia coli ATCC 8739 (WDCM 00012): none - Staphylococcus aureus ATCC 6538 (WDCM 00032): none - Candida albicans ATCC 10231 (WDCM 00054): good - Saccharomyces cerevisiae ATCC 9763 (WDCM 00058): good - Pseudomonas aeruginosa ATCC 9027 (WDCM 00026): inhibited - Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053): goodIncubation: 48 hrs.; 28 °C; aerobic; Aspergillus up to 72 hrs

Application

This medium is recommended for the selective isolation and enumeration of yeasts and moulds from environmental materials and foodstuffs. Envirocheck contact slides can be used for both surface and liquid testing in industrial and environmental monitoring.

General description

(Yeasts and Molds) Envirocheck^™

Envirocheck Contact Yeasts and Moulds, a semi-quantitative detection of microorganisms on surfaces and in liquids, are flexible paddles that are coated with Tryptic soy agar to determine the yeasts and moulds from samples. Slide A is coated with CASO (Tryptic Soy) Agar containing 0.05% Triphenyl Tetrazolium chloride (TTC). The growth of bacteria is indicated by red colonies due to the formation of red formazan dye when TTC is reduced and Slide B is coated with Rose Bengal Chloramphenicol Agar. Chloramphenicol inhibits the growth of bacteria additionally to Rose Bengal. Rose Bengal reduces the size and height of mould colonies so that slow-growing fungi are not overgrown by more rapidly growing species.

Legal Information

ENVIROCHECK is a trademark of Merck KGaA, Darmstadt, Germany

Packaging

10 units in Fibre case