Application

Histone is suitable for use:as a positive control in the in vitro kinase assay to examine the ability of Cdc2-cyclin B1 to phosphorylate GST-CD-4 proteinin Cdk5 activity assayin histone H1 kinase assayas a Poly(ADP-ribose) (PAR) acceptor in the assessment of in vitro PAR polymerase activityas an acceptor of ³²P from [γ-³²P]ATP in order to measure protein kinase activity of lysate from small and large luteal cells

Biochem/physiol Actions

Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA. Based on the fractions, the molar mass of histones varies between 11-21kDa. Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich. H1 acts as a link between "beads" (nu bodies) on the chromatin chain.

Features and Benefits

Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.

Other Notes

Characterized as mainly subgroup f₁ by SDS-PAGE

Packaging

25, 100, 250 mg in poly bottle

Preparation Note

Lysine-rich fraction as isolated and described by de Nooij, E. and Westenbrink, H.G., Biochim. Biophys. Acta, 62, 608 (1962).