Application
Discovery® RP-AmideC16 HPLC column may be used for the separation of:Phenolic compounds, purine alkaloids and theanine in Camellia species by high performance liquid chromatography (HPLC) equipped with photodiode array detector (PDA). Proprietary pharmaceutical compounds using HPLC combined with computer-assisted screening and Plackett−Burman experimental design. Hydrophilic bases by LC with principal component analysis (PCA). 2,4-Dinitrophenylhydrazone derivatives by HPLC equipped with ultraviolet (UV) spectrometric detection.
Discovery RP-AmideC16 HPLC Column may be used for the separation of:Impurities in drugs such as closantel, levamisole and azaconazole during their determination by high performance liquid chromatography-diode array detection (HPLC-DAD).Vitamin B6 compounds in food samples during their determination using LC coupled with fluorescence detection (FLD).Vitamin C and other fat soluble vitamins in mature seeds of Lathyrus L. taxa during their identification using HPLC with ultraviolet (UV) detection.Zidovudine in combination with other pharmaceuticals in intravenous fluid mixtures during the determination using HPLC combined with photodiode array (PDA) detector.
Features and Benefits
Excellent retention and resolution of polar compounds Unique selectivity compared to C18 Excellent peak shape and efficiency Less hydrophobic than C18 columns Compatible with 100% aqueous mobile phases
General description
Discovery® RP-AmideC16 HPLC Column consists of a stationary phase made up of high-purity C16-silica that includes a polar-embedded amide function attached to the silica surface with a propyl group.
Legal Information
Discovery is a registered trademark of Sigma-Aldrich Co. LLC
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