Application

For use in Southern hybridizations.

Deoxyribonucleic acid from human placenta has been used:to compare the efficiency of DNA quantification methods as a standard in real-time PCR analysis of DNA samples from bladder cancer cell linesas an internal control to estimate the degree of fragmentation of circulating DNAfor restriction digest analysis to identify low copy repeat regions of human chromosome 22q11to compare the 70-bp P5 exon sequence between DNA of different human and primate speciesto calculate copy number of genes, relative to normal human tissuein PCR reactions

General description

Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.

Recommended products

Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 µg/ml, DNA tends to absorb onto the surfaces of plastic tubes.