4-Methylumbelliferyl N-acetyl-beta-D-glucosaminide, suitable for fluorescence, >=99.0% (TLC)

Code: 69585-250MG D2-231

Application

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide has been used as a fluorogenic substrate in the acidic chitinase activity assay. It has also been used to...


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Application

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide has been used as a fluorogenic substrate in the acidic chitinase activity assay. It has also been used to measure the total activity of β-N-acetylhexosaminidase (NAGase) in water sample filtrates.

Biochem/physiol Actions

4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MUF-NAG) is a synthetic uncharged fluorogenic substrate for hexosaminidases. It consists of chitin monomer (N-acetyl-β-D-glucosaminide) and 4-methylumbelliferone (7-hydroxy-4-methylcoumarin) (4-MUF). 4-MUF-NAG is used for the detection and estimation of fungal growth by measuring the β-N-acetylhexosaminidase (NAGase) activity.

Other Notes

Assay of β-N-acetylhexosaminidase

assay≥99.0% (TLC)
fluorescenceλex 365 nm; λem 445 nm in aqueous buffer pH 5.0 (after cleavage by β-N-acetylglucosaminidase), λex 317 nm (pH 10.0)
formpowder
impurities≤0.1% free 4-methylumbelliferone
InChI keyPAVCYMSNMRWMAK-DMYIEBNJSA-N
InChI1S/C18H21NO8.2H2O/c1-8-5-14(22)26-12-6-10(3-4-11(8)12)25-18-15(19-9(2)21)17(24)16(23)13(7-20)27-18;;/h3-6,13,15-18,20,23-24H,7H2,1-2H3,(H,19,21);2*1H2/t13-,15-,16-,17-,18-;;/m1../s1
Quality Level100
SMILES string[H]O[H].[H]O[H].CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc3C(C)=CC(=O)Oc3c2
solubilityDMF: 20 mg/mL, clear, colorless
storage temp.−20°C
suitabilitysuitable for fluorescence
Code
Description
Unit Size
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69585-1G
4-Methylumbelliferyl N-acetyl-beta-D-glucosaminide, suitable for fluorescence, >=99.0% (TLC)

Unit:1G
List Price: £747.00
Source:List Price
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Cas Number37067-30-4
This product has met the following criteria: