Application

Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.

Suitable for: Preparing nucleic acids for hybridization Hybridization buffer for Northern and Southern blots Washing buffer for Northern and Southern blots

Components

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

Features and Benefits

Multi-purpose use in hybridizations Easily reconstituted and diluted to any concentration needed

General description

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.