Application

PCR 100 bp Low Ladder has been used:in agarose gel electrophoresis as a standard DNA marker to obtain DNA fragments of about 240 bp for polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) analysisto visualize DNA from enzyme digested PCR mixtureto estimate the molecular weight of the PCR product fragments

Components

Sigma’;s PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

General description

Sigma’;s PCR 100 bp Low Marker contains 10 dsDNA fragments from 100 – 1,000 bases, evenly spaced in 100 bp repeats. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

The PCR 100 bp low ladder has been specially designed for the size determination of polymerase chain reaction (PCR) generated DNA fragments.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Contains 10 bands, 100–1,000 bp in exact 100 bp spaced (ladder) recombinant repeats.