Application

DNA Molecular Weight Marker XIII (50 bp ladder) allows accurate sizing of DNA fragments generated by PCR or restriction digest, followed by separation on agarose gels. DNA Molecular Weight Marker XIII (50 bp ladder) can be used in conjunction with DNA Molecular Weight Marker XIV (100 bp ladder) for precise size determination.The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g.,³²P-dTTP or ³²P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.

DNA Molecular Weight Marker for agarose gels; simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:Restriction digestsPCRRT-PCR

General description

Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases. Size Range: 50 to 750 bp and an additional band of 2642 bp

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Ready-to-use solution, 250µg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).

Preparation Note

Storage conditions (working solution): 2 to 8 °COnce thawed we recommend further storage at 2 to 8 °C.

Sequence

The mixture contains 15 double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, and an additional band of 2642 bp. Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. The 250- and 500-bp banding pattern is two to three times brighter.

Unit Definition

50 µg = 1A₂₆₀ unit