Application

DNA Molecular Weight Marker VIII is used for the size determination of DNA in agarose gels by:Restriction digests PCRRT-PCRNote: Use this marker to size DNA fragments between 19 and 1,114 bp.This marker is also available in DIG-labeled form (DNA Molecular Weight Marker VIII, DIG-labeled).

Features and Benefits

Fragment mixture prepared by cleavage of pUCBM21 DNA with Hpa II, and pUCBM21 DNA with Dra I and Hind III.ContentsReady-to-use solution, 250µg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).

General description

DNA Molecular Weight Marker VIII contains a mixture of 17 DNA fragments.The product is also available as DNA Molecular Weight Marker VIII, Digoxigenin-labeled.Size range: 19 to 1114 bp

Other Notes

Use this marker to size DNA fragments between 19 and 1,114 bp.This marker is also available in DIG-labeled form (DNA Molecular Weight Marker VIII, DIG-labeled).

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Storage conditions (working solution): 2 to 8 °COnce thawed we recommend further storage at 2 to 8 °C.

Sequence

The mixture contains 17 DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 19, 26, 34/34, 37, 67, 110, 124, 147, 190, 242, 320, 404, 489, 501, 692, 900, and 1114 bp.Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Unit Definition

50 µg = 1A₂₆₀ unit