Application

DNA Molecular Weight Marker VI is used for the size determination of DNA in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:Restriction digestsPCRRT-PCR

General description

DNA Molecular Weight Marker VI is a fragment mixture prepared by cleavage of pBR328 DNA with restriction endonucleases Bgl I and pBR328 DNA digested with Hinf I. It has a size range of 0.15 to 2.1kbp.The product is also available as DNA Molecular Weight Marker VI, Digoxigenin-labeled.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Ready-to-use solution, 250µg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).

Preparation Note

Storage conditions (working solution): 2 to 8 °COnce thawed we recommend further storage at 2 to 8 °C.

Sequence

As determined by computer analysis of the pBR328 sequence, the mixture contains 15 DNA fragments with the following base pair lengths (1 base pair = 660 daltons) occuring once: 220, 394, 453, 517, 653, 1033, 1230, 1766, 2176 and fragments 154,234, and 298 occuring twice as a result of the digestion of pBR328 with both Bgl I and Hinf I.Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Specificity

Specificity: Product has 5′-phosphorylated ends (restriction fragments).Restriction site: Claevage sites and corresponding fragment lengths of pBR328:- Bgl I cut sites at: 935, 1169, 2399, 4575, 4809 => creating following fragment sizes, respectively: 234 bp, 1230 bp, 2176 bp, 234 bp, 1033 bp.- Hinf I cut sites at: 632, 852, 1006, 1304, 1757, 2274, 4040, 4434, 4732, 4886. =>creating following fragment sizes, respectively: 220 bp, 154 bp, 298 bp, 453 bp, 517 bp, 1766 bp, 394 bp, 298 bp, 154 bp, 653 bp

Unit Definition

50 µg = 1A₂₆₀ unit