Application

Poly-D-Lysine & Laminin (2.5 ML) has been used in vitro as inductive agents to stimulate Y79 cells to express both neuronal and glial characteristics.

General description

Poly-D-Lysine and Laminin solution is prepared in phosphate-buffered saline and sterile filtered. It is used to coat tissue culture ware to culture surface ratio of 0.25 ml/cm². Tissue culture ware is coated at room temperature for one hour and carefully aspirate the solution. After wash two times with PBS, coated tissue culture ware may be used immediately or air-dried and stored at 4^oC for up to one week.